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The gene-synthesized PSD95-(SH3-GK) (427-724) domain was purified using Ni 2+ -affinity chromatography (HiTrap IMAC FF, Cytiva), followed by thrombin digestion and anion-exchange chromatography (HiTrap Q FF, Cytiva)

Peptide bond hydrolysis accelerates logarithmically with temperature

However, these products (as well as others with demonstrated lipid-lowering properties) may provide a good solution for: (1) statin-ineligible patients receiving early pharmacotherapy of dyslipidaemia, (2) patients declining statin therapy, (3) statin-intolerant patients, and (4) all those who (both in primary and secondary prevention), despite available treatment, still fail to achieve the treatment goal

As a result, glutamine metabolism is an important potential target for cancer therapy