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It is also important that the vials should not be exposed to extreme temperatures or direct light, so that the efficacy of their preservative, as well as the reconstituted medications, remains intact

The cells were left to differentiate into MDMs for 7 days, then washed with PBS, treated with 5 mM PBS/EDTA at 37C for 20 min, harvested gently with a cell scraper, counted and replated on 96- or 6-well trays at 1104 and 1106 cells per well, respectively, as described previously (31)

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The bilayer is composed of 82.05% Phosphatidylcholine (PC) and 10.82% Phosphatidylethanolamine (PE), yielding a total phospholipid content of 93%, with over 75% forming uniform, high-integrity liposomal structures