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RNA isolation, reverse transcription, and qRT-PCR analysis Total RNA from STC-1 cells was extracted using an RNA miniprep kit (Takara, 9108)

The capillary voltage was set in a range of 22 to 25 V, the spray voltage was between 3.00 and 4.50 kV, and a capillary temperature of 180 C was employed

2023 Case control Study 243 patients 6 months Med Diet vs Green MED NAFLD Plant protein group showed better result than meat group 3 Ristic-medic et al 2020 RCT 24 Obese patients 3 months Med Diet vs Low fat group NAFLD Significant weight loss, liver enzymes, insulin levels in Med gr oup compare to Low-fat group 4 Tsaban et al

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