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The researchers injected conjugated gold nanoparticles into mice that had been given an excessive dose of acetaminophen (APAP)

Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]

We have described liver organoid models 4,5,6 (recently renamed as intrahepatic cholangiocyte organoids 18 ) in which cholangiocytes/ductal cells can be expanded long term in culture

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