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IRAP/AT 4 receptor (/) mice had comparable baseline blood pressure and CBF, and comparable responses to Ang IV as their corresponding wild-type mice, confirming that the putative IRAP/AT 4 receptor is not involved in the pressor and renal hemodynamic effects of Ang IV

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For molecular analysis, immunofluorescence, and immunohistochemistry techniques were extensively used on human testis, mouse testis, and TM3 cells to visualize the expression and subcellular localization of key proteins