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In addition, the purity of raw materials, formulation concentrations, and delivery systems vary between studies

Media Attributions Figure 11.1.1 Steven Earle, CC BY SA 4.0, after Water Cycle by Ingwik, CC BY SA 3.0, via Wikimedia Commons, Figure 11.1.2 Steven Earle, CC BY 4.0 Figure 11.1.3 Modified by Steven Earle, from Mackenzie River with Heavy Sediment Load by Pierre Markuse, 2018, CC BY 2.0 via Flickr,

Surface expression was measured on the Attune NxT Flow Cytometer, data were analyzed using FlowJo 10.8.1 and expressed as % positivity and MFI

Purity of cagrilintide was determined using the following reversed phase high performance liquid chromatography (RP-HPLC) where the samples were analysed using a Kinetex C18, 2.6 pm column (4.6 x 150 mm) with a gradient elution of eluent A consisting of 90% v/v 0.09 M phosphate solution, pH 3.6 and 10% v/v acetonitrile, and eluent B consisting of 60% v/v acetonitrile and 20% v/v isopropanol