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doi:10.1001/jama.2019.2942 Pratley RE, Aroda VR, Lingvay I, et al

Results vary based on individual metabolism, diet, and adherence to the program

Millions of people try to lose weight every year to combat the adverse effects of weight gain, but many of those people are unsuccessful

The comparative threshold cycle (Ct) method was used to measure the relative expression, where 2 Ct represents the fold change in expression, as previously described (Livak and Schmittgen, 2001)