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Metabolic role of dipeptidyl peptidase 4 (DPP4) in primary human (pre)adipocytes

The cell pellet was thawed, homogenized, and lysed in a buffer containing 20 mM HEPES pH 7.5, 50 mM NaCl, 2 mM MgCl 2 , and complete protease inhibitor cocktail tablet (Roche), and the membranes were collected by centrifugation at 40,000 g

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During the exposure of riboflavin to UVA, singlet oxygen molecules are generated, which induces covalent bonding between amino groups of collagen fibrils, and thus strengthens tissue stiffness 136