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Liver were snapped freezing under liquid nitrogen and homogenized in QIAzol reagent (Qiagen) using Tissue Lyser (Qiagen)

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In 2020, a noteworthy study reported that 7,8-dimethoxycoumarin increased melanogenesis and TYR activity in B16F10 cells by upregulating the expression of TRP-1, tyrosinase-related protein 2 (TRP-2), and MITF, and interfered with the phosphorylation of ERK in the MAPK pathway [120]