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The Nrf2 under physiological ROS levels is bound to Keap1, is constantly ubiquitinated via Cul3 E3 ubiquitin ligase, and is subsequently degraded by the proteasome [24]

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Statistical analysis For animal studies, sample sizes ( n = 5 per group, 3 in the tirzepatide-treated group) were determined based on prior in-house experience and previous studies using similar mouse models to ensure adequate power to detect biologically relevant effects