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Jul-Dec 2013;19(2):49-55

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[DOI] [PMC free article] [PubMed] [Google Scholar] 177.Ning C., Li Y.-Y., Wang Y., Han G.-C., Wang R.-X., Xiao H., Li X.-Y., Hou C.-M., Ma Y.-F., Sheng D.-S., et al

Once the culture has been induced with IPTG, do not allow the cells to grow for extended periods of time, as cell lysis can occur and release proteases into the cytoplasm that could degrade the fusion protein