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At an OD 600 of 0.60.7, expression was induced with 1 mM IPTG and the culture was continued up to an OD 600 of 3.0 where the cells were harvested at the end of the exponential growth phase by centrifugation (10 min, 3,220 g , 4C)

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Swim from the Atlantic to the Caribbean, explore caves on the Caribbean side, enjoy a beach BBQ on the Atlantic side, and for the more adventurous, trek to the lighthouse

Comparative Biochemistry and Physiology Part A , 234 , 3649