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Add-On Treatment with N-Acetylcysteine for Bipolar Depression: A 24-Week Randomized Double-Blind Parallel Group Placebo-Controlled Multicentre Trial (NACOS-Study Protocol) Int

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The protein was eluted with buffer containing 20 mM HEPES, pH 7.5, 500 mM NaCl, 0.1% (w/v) DDM, 0.02% (w/v) CHS, 400 mM imidazole and 1 M PF-05190457 and loaded onto an anti-Flag M1 antibody resin after adding 2 mM CaCl 2