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Pigment can recur with sun or hormones

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After 30 min at 37C cells (ring-enriched, trophozoite-enriched or non-infected RBCs) were washed 3 times with PBS-G, adjusted to 50% hematocrit and analyzed by FACS for surface-bound IgG and complement C3c fragment, and utilized for the phagocytosis assays with THP-1 cells and human peripheral monocytes