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After completion of the isoelectric focusing, the strip was equilibrated in the equilibration solution as described by Barraclough [37] and placed on a polyacrylamide gel for SDS-PAGE with a Protean II unit (Bio-Rad, USA) at a constant voltage (300 V)

doi: 10.1080/21691401.2017.1337024

The remodeled microbiome composition further optimizes the integrity and permeability of the intestinal barrier via Occludin, ZO-1, Claudin-2, and Mucin 2 (MUC2), at the same time suppressing the activity of M1-type macrophages, thereby improving the clinical indices of bone health, including BMD, BV/TV, and Tb

The device is designed to minimize discomfort with its ultra-fine needles.