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Each larva was dissected in phosphate buffered saline (PBS) under a stereomicroscope (ZEISS Stemi 508, ZEISS, Oberkochen, Germany) and checked for the parasitoid immature stages (a layer of hemocytes surrounding the parasitoid egg or larva) and melanisation (black/brown hemocyte layers around the parasitoid egg) status

As the body's master antioxidant, glutathione plays indispensable roles in detoxification, immune function, cellular protection, and numerous other physiological processes essential for health and longevity

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Epigenetic disruption of this enzyme system exposes the fetal brain to an excess of cortisol with possible extensive long-term destructive effects [64,65]