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Nevertheless, preliminary studies that find the right non-toxic dosage is important, whether in vitro or in vivo as screening for study feasibility, but still, further human study is required to determine the dose safety and efficacy (Stohs and Hartman, 2015)

Although exosome precipitation is simple and exosome isolation can be completed in a single step, inconsistent yields, time-consuming sampling and cleaning processes, and a shortage of a suitable targeted isolation technique undoubtedly compromise the high quality of the separated EXOs, compromising subsequent analyses (Wang et al., 2021

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