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Adult mouse astrocyte isolation Brain tissues were aseptically removed, minced and enzymatically digested in HBSS (Gibco, 14175103) containing 50 mg ml 1 collagenase D (Sigma-Aldrich, 11088858001), 100 g ml 1 TLCK trypsin inhibitor (Sigma-Aldrich, T9378), 100 U l 1 DNase I (Sigma-Aldrich, D5025), and HEPES pH 7.2 (Gibco, 15630080) for 1 h at 23 C with shaking

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In 2012, our lab identified several families with a form of autism caused by mutations in BCKDK, a gene involved in the metabolism of branched-chain amino acids

Third-Party Testing Verification: High-quality providers conduct or obtain third-party testing verifying potency (actual glutathione concentration matches label claims), sterility (absence of bacterial or fungal contamination), pH appropriateness (formulated for tissue compatibility), and absence of contaminants including heavy metals and endotoxins