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DNA isolation from MACS-sorted FoB and MZB was performed with NucleoSpin 96 Tissue kit (Macherey-Nagel 740454.4)
HepaRG cells (8000 cells/25 L/well) or PHHs (Hu8163, 17,500 cells/25 L/well) were seeded in collagen I-coated 384-well white flat-bottom plates (Corning, catalog: 354665)
Men are also more likely to develop conditions associated with reduced glutathione or higher oxidative stress, including, More visceral fat than women, which drives insulin resistance 49 Higher rates of sleep apnea, another contributor to oxidative stress 50 Cardiovascular disease, which occurs about 710 years earlier in men than in women on average 51 As estrogen declines through perimenopause and menopause for womenand remains lower in men overalldifferences in glutathione activity likely reflect hormonal influence as much as sex-based biology, Tennant says
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