ELISA-based binding assay: Clear bottom 384-well plates (high-binding #781061, Greiner, Kremsmnster, Austria), nonfat dry milk (#T145.3, CarlRoth, Karlsruhe, Germany), anti-tetramethylrhodamine (TRITC) antibody (#A6397, Invitrogen, Waltham, MA, USA), HRP-conjugated rabbit secondary antibody (#7074S/#7076S, Cell Signaling, Danvers, MA, USA), 3,3,5,5-tetramethylbenzidine substrate (#ES022, Merck, Darmstadt, Germany)
Dysregulated cholesterol homeostasis results in resistance to ferroptosis increasing tumorigenicity and metastasis in cancer
Setria Glutathione has been shown to decrease oxidative stress, increase natural killer cells, and significantly boost glutathione levels
Zinc Essential mineral that supports immune function and contributes to skin, hair, and nail health