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To assess whether RT probe binds to any proteins or reacts with free cysteine residues in some proteins that may interfere with the final ratio readouts, we incubated RT with cell lysate, which was dialyzed using a 3 K cut-off membrane to remove all small molecules including GSH, and observed no significant changes in fluorescent signal ratios even at the highest lysate concentration shown (protein concentration is 36 mg ml 1 based on a BCA assay), suggesting that there is no non-specific interactions or reactions between cellular proteins and RT which could significantly change the ratio readouts (Fig

We wondered if there were time-specific functional changes along biofilm development or if biofilm physiological state is triggered just by upregulating and downregulating a number of genes

2010) for validation and/or optimization of the high-penalty parameters and charges, the energy of each system was minimized (Pan and Roux 2008) to remove any steric clashes or unusual geometry by steepest descent algorithm using the input parameter EM file (Hess et al

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