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Attached THP-1 cells were stimulated with 100 ng ml 1 lipopolysaccharide (Sigma) for 3 h and treated with DMSO, GSTO1 inhibitors or Glyburide (Enzo Life sciences) in serum-free media for 1.5 h followed by addition of 5 mM ATP (Sigma) for 30 min

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The networks showed that the major potential targeting pathways of (-)-Vestitol included Neuroactive ligand-receptor interaction pathway, Calcium signaling pathway, Metabolic pathways, Alzheimer disease, Estrogen signaling pathway, PI3K-Akt signaling pathway, and Immune system