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However, this method has also a disadvantage such as high production costs of (R)-4-chloro-3-hydroxybutyrate derivate, and a difficulty of application thereof to industrial-scale production in that the reaction must be carried out under high- temperature and high-pressure conditions
For samples derived from media containing 13 C glucose or 13 C glutamine, methoximation was followed by silylation by adding 80 l of MTBSTFA (with 1% TBDMS, Thermo) and incubation for 60 min at 70 C
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