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Since it was practically difficult to culture HUVEC for a long period, we cultured HUVEC for 35 days under increased glucose and/or cholesterol levels and then evaluated GLP-1 receptor expression levels

References Arini A, Daffe G, Gonzalez P, Feurtet-Mazel A, Baudrimont M (2014) Detoxification and recovery capacities of Corbicula fluminea after an industrial metal contamination (Cd and Zn): a one-year depuration experiment