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The resulting single-stranded cDNAs were used as templates in PCR reactions using gene-specific primers to amplify TGR (forward 5-CTATTTCCGTAGACGTCTGT-3 and reverse 5-AATACAGTTTCCTTCCCGTT-3) or GAPDH (forward 5-GTTTTGGTCGTATCGGGAGA-3 and reverse 5-ATGCGTTAGAAACCACGGAC-3)

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But these data gaps further underscore the need to study this population, as currently, there is "limited or no clinical guidance" on the use of GLP-1s prior to conception, the commentary authors wrote

[DOI] [PubMed] [Google Scholar] 10.Mahaffey K.W., Jardine M.J., Bompoint S., et al