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Liquid-chromatography mass spectrometry (LC-MS) is considered to be a gold standard for redox metabolite measurements 41

All embryos were cultured using sequential culture medium (Vitrolife, Gothenburg, Sweden) in incubators at 37C under 5% O 2 , 6% CO 2 , and 89% N 2 conditions with high moisture until day 6

In addition to weight reduction, treatment with mazdutide was associated with significant improvements in metabolic parameters, including reductions in glycated hemoglobin (HbA1c), fasting plasma glucose, blood pressure, and lipid profiles in individuals with type 2 diabetes mellitus