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Proteins were digested into peptides using a high-throughput automated version of the protein aggregation capture workflow 52 on the KingFisher Flex Purification System (Thermo Fisher Scientific)

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When transplanted via the tail vein into cyclophosphamide-induced POI model mice, MenSCs regulate follicular development, restore estrous cyclicity, reduce ovarian apoptosis, and maintain microenvironmental homeostasis via ECM-dependent FAK/AKT signaling activation (Feng et al., 2019)