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To further verify the role of DNMT1 in HOXA2 promoter methylation, DNMT1 siRNA was used to transfect HK-2 cells

15 The large range in recurrence rates found in the literature is likely due to differences in study designs (e.g., differing definitions of recurrence/relapse, isotretinoin doses, and duration of treatment and follow-up periods)

First, the non-randomized, single-center design may limit the generalizability of findings to broader adolescent populations or different surgical practices