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After inspection of the apo-OsDHAR structure, we predicted that the highly conserved Cys20 residue acts as the catalytic nucleophile and found that this residue is located at the cleft between the N- and C-terminal subdomains

[48] Analysis results of racemic ethyl 4-chloro-3-hydroxybutyrate are as follows: [49] 1 H-NMR (CDCl 3 ) = 1.28 (3H,t), 2.62 (2H,d), 3.53 (lH,br), [50] 3.60 (2H,d), 4.20 (2H,q), 4.33 (lH,m) [51] [52] Example 3: Preparation of ethyl (RV4-chloro-3-hvdroxybutyrate (4) [53] 40 g (10% w/v) of racemic ethyl 4-chloro-3-hydroxybutyrate was added to a flask containing 360 mL of 0.1M potassium phosphate buffer (pH 8.0) to which 4 g (1% w/ v) of immobilized lipase Novozyme 435 was then added, followed by reacting at 30 0 C

Buffer capacity is the ability of a buffer to resist changes in pH

Even more striking, 63% of those on the 15mg dose lost at least 20% of their body weight