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Free Radic Biol Med (2000) 28(4):62535.10.1016/S0891-5849(99)00275-0 15 KirlinWGCaiJThompsonSADiazDKavanaghTJJonesDP

A T/C polymorphism in the GPX4 3UTR affects the selenoprotein expression pattern and cell viability in transfected Caco-2 cells

The supernatants were mixed with 0.1% acetic acid (6 mL) and loaded onto solid-phase lipid extraction cartridge, which was washed with 15% methanol, 0.1% formic acid (2 mL), and the lipids were eluted with 500 L of anhydrous methanol and subsequently with 500 L of acetonitrile

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