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When it comes to what goes in your e-cigarette, your e-liquid will typically contain pharmaceutical grade nicotine, also known as freebase nicotine, that has been extracted from tobacco via a chemical process to distil a final product that is up to 99.9% pure

Thus, mainstream smoke depends on the chemical composition of the combustible portion of the cigarette near the periphery of the rod, whereas chemicals at higher concentrations in the central portion of the rod have higher levels in sidestream smoke than in mainstream smoke (Johnson 1977)

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The process required a variety of research tools along the way, including methods of manipulating organs, nerves, and cell receptors