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The engineered CAR used the scFv regions previously described 42,54 but with the CD8a hinge/TM domains and 4-1BB costimulatory domain
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However, regarding the nasal spray, microparticles are not expected to penetrate the mucin barrier that protects the respiratory epithelial cells (3941), and so this now motivates a deeper physicochemical evaluation of niclosamide and especially how to increase its concentration in solution using simple buffers to access the epithelial cells
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