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10.1074/jbc.M112.402776 79 MathieuY.ProsperP.FavierF.HarvengtL.DidierjeanC.JacquotJ

The IRC, or infrared coagulator, contains a light source at its tip that applies heat to the hemorrhoid columns base

It is a metabolic stressor one that actively worsens insulin sensitivity, elevates cortisol, disrupts GH secretion, upregulates hunger hormones, and promotes visceral fat accumulation

After 24 hpi, each well was washed, fixed with 4% Histofix (Roth), mounted with ProLong Gold antifade mountant (Thermo Fisher Scientific), and visualized at 64 magnification with an Axio Observer microscope (Carl Zeiss Microscopy) using bright field and fluorescence channels (596/621 nm)