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4.How frequently is the medication used

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HEK293T cells (ATCC) were transfected with pCAGGS-PRDX-HA using Lipofectamine 2000 (Thermo Fisher Science) and cultured in DMEM supplemented with 10% FBS for 5 days

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Contaminating genomic DNA was eliminated by the inclusion of a deoxyribonuclease treatment step (deoxyribonuclease I at 8 U per 100 L of eluate) ( 260 to A 280 ratio) using a Nanodrop 1000 instrument

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Corciulo, C

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