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In isolated sinus nodes, GLP-1 shortens action potential cycle length and shifts the site of earliest activation independently of HCN channel conduction To further investigate the electrophysiological effect of GLP-1 on the sinus node, isolated porcine sinus nodes (see Supplementary material online, Figure S6A ) were exposed to continuous superfusion with GLP-1, ivabradine, or ivabradine + GLP-1

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