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The molecular masses of all protein species were monitored by liquid chromatographytime-of-flight mass spectrometry (LC/MS TOF 6230B, Agilent, Santa Clara, CA, USA) using the MassHunter Qualitative Analysis 12.0 software (Agilent, Santa Clara, CA)

In a randomized controlled trial, treatment with orlistat 120 mg three times daily resulted in a mean BMI reduction of 0.55 kg/m 2 , whereas the placebo group experienced a mean increase of 0.31 kg/m 2 [58]

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