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Breast cancer screening involves the observation of precancerous lesions and infiltrating tumours at early stages in females who do not present symptoms, with qualitative and economic methods

CEN.PK 102-3A and CEN.PK 113-6B containing the overexpression construct for GSH1 were subsequently transformed with YIplac128- TPIp-CYS3-CYC1t previously linearized in LEU2 with ClaI and selected on YNB agar plates with supplementation of amino acids when applicable

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The supernatants were analyzed using the Mouse Insulin ELISA Kit (Mercodia, Sweden)