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International Union of Pure and Applied Chemistry and International Federation of Clinical Chemistry

10.1371/journal.pgen.1002573 203 NilssonR.JainM.MadhusudhanN.SheppardN

Bhavana Shetty Dietician 7 Years DDHN & Masters in Clinical Nutrition & Dietetics Bangalore Apollo Sugar Clinic, Seetha circle bangalore, Bangalore

A minimum of 5 non-overlapping images were captured per wells and ImageJ was used to calculate corrected total cell fluorescence (CTCF): CTCF = Integrated Density (Area of selected cell x Mean fluorescence of background readings) 21