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The separation was achieved using an RP C8 column, with heptafluorobutyric acid as an ion-pairing reagent to optimize retention, particularly since these compounds are cations under acidic conditions

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But there are differences in how theyre taken

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10.3389/fncel.2023.1277268 15 CaoH.ZuoC.HuangY.ZhuL.ZhaoJ.YangY.et al (2021)

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