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For cell number measurements in 6-well plates, the medium was aspirated, the wells were rinsed with PBS and the cells were detached by incubating with 500 l of 0.25% trypsin (Thermo Scientific, 25200056) for 20 min at 37 C

If you see a bubble in the syringe barrel, flick the syringe gently until the bubble rises to the top, then push the plunger slightly to expel it before measuring your dose

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Further, we show that this reduction in incidence is also true in patients with both CP and T2DM