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Shaking the vial (denatures peptides), injecting water too quickly (creates foam), using the wrong volume because the math was done by hand instead of a peptide dosage calculator, storing reconstituted peptides at room temperature, freeze-thaw cycles, and failing to label vials with concentration and date

Library preparation and sequencing were performed, followed by the trimming of sequence reads using fastp v.0.23.13 to remove potential adaptor sequences and low-quality nucleotides

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more than 4 cm length 64890 Nerve graft (includes obtaining graft), single strand, hand or foot