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Additionally, the researchers examined the effects injection of melanotan II had on NPY mRNA expression after twice daily injections from postnatal days 5-10 and 10-15 as well as the effects the treatment had on the activation of the central c-Fos gene which was tested 90 minutes after injection of melanotan II [2]

Standard curve samples were prepared in mcubation buffer and 500 SL added to 500 ice-cold acetonitrile with internal standard

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