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Materials and methods Cell culture GLUTag cells [24] were cultured in DMEM (5.5 mmol/l glucose), as described previously [14]

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D., Valentini, G., Bianchi, G., Biasi, G., Cacace, E., Adami, S

Chromatographic Methods (HPLC, LC-MS): Used for separating, identifying, and quantifying methionine cycle intermediates, particularly folate derivatives and amino acid metabolites, ensuring precise metabolic profiling